An optimised whole mount in situ hybridisation protocol for the mollusc Lymnaea stagnalis
نویسندگان
چکیده
منابع مشابه
A neuronal network from the mollusc Lymnaea stagnalis.
The morphology, electrophysiology, and synaptic inputs of a ventrally located neuronal network from the CNS of the pond snail Lymnaea stagnalis was investigated. Three large, previously identified neurons [55] known as right parietal ventral one, two, and three (RPV1,2,&3) were found to be electrically coupled to one another. Coupling between either RPV1&2 or RPV1&3 was weak while coupling betw...
متن کاملBehavioural evidence for a sleep-like quiescent state in a pulmonate mollusc, Lymnaea stagnalis (Linnaeus).
The objective of this study was to determine whether the great pond snail, Lymnaea stagnalis, expresses a sleep-like behavioural state. We found that snails spontaneously enter a relatively brief (22±1 min) quiescent state characterized by postural relaxation of the foot, mantle and tentacles, and cessation of radula rasping. Quiescence was reversed ('aroused') by appetitive (sucrose solution) ...
متن کاملMedium-Throughput Processing of Whole Mount In Situ Hybridisation Experiments into Gene Expression Domains
Understanding the function and evolution of developmental regulatory networks requires the characterisation and quantification of spatio-temporal gene expression patterns across a range of systems and species. However, most high-throughput methods to measure the dynamics of gene expression do not preserve the detailed spatial information needed in this context. For this reason, quantification m...
متن کاملEffect of acute temperature change on lung respiration of the mollusc Lymnaea stagnalis
1. Temperature-dependent effects on respiratory behaviour as well as the corresponding temperature-dependent activities of identified neurons within the respiratory network of the pulmonate snail Lymnaea stagnalis were investigated. 2. Lymnaea lung ventilation terminated at low temperatures (under 10 1C) while temperature elevation increased ventilation rates. The respiratory central pattern ge...
متن کاملPredator detection in Lymnaea stagnalis.
Laboratory-reared Lymnaea are capable of detecting and responding to the scent of a crayfish predator. The present investigation is a first attempt to characterize multiple stress-related behavioural responses resulting from predator detection and to depict the neurophysiological correlates of one of these illustrated behaviours. Snails respond to crayfish effluent (CE) by increasing the follow...
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ژورنال
عنوان ژورنال: BMC Developmental Biology
سال: 2015
ISSN: 1471-213X
DOI: 10.1186/s12861-015-0068-7